Noninvasive Oxygen Monitoring in Three-Dimensional Tissue Cultures Under Static and Dynamic Culture Conditions
نویسندگان
چکیده
We present a new method for noninvasive real-time oxygen measurement inside three-dimensional tissue-engineered cell constructs in static and dynamic culture settings in a laminar flow bioreactor. The OPAL system (optical oxygen measurement system) determines the oxygen-dependent phosphorescence lifetime of spherical microprobes and uses a two-frequency phase-modulation technique, which fades out the interference of background fluorescence from the cell carrier and culture medium. Higher cell densities in the centrum of the scaffolds correlated with lower values of oxygen concentration obtained with the OPAL system. When scaffolds were placed in the bioreactor, higher oxygen values were measured compared to statically cultured scaffolds in a Petri dish, which were significantly different at day 1-3 of culture. This technique allows the use of signal-weak microprobes in biological environments and monitors the culture process inside a bioreactor.
منابع مشابه
Three dimensional static and dynamic analysis of thick plates by the meshless local Petrov-Galerkin (MLPG) method under different loading conditions
In this paper, three dimensional (3D) static and dynamic analysis of thick plates based on the Meshless Local Petrov-Galerkin (MLPG) is presented. Using the kinematics of a three-dimensional continuum, the local weak form of the equilibrium equations is derived. A weak formulation for the set of governing equations is transformed into local integral equations on local sub-domains by using a uni...
متن کاملThe Effect of Rosmarinic Acid in Neural Differentiation of Wartons Jelly-derived Mesenchymal Stem Cells in Two Dimensional and Three Dimensional Cultures using Chitosan-based Hydrogel
Numerous studies have shown the positive effects of rosmarinic acid on the nervous system. Rosmarinic acid as a herbal compound with anti-inflammatory effects can prevent the destructive effect of inflammation on the nervous system. Furthermore, various studies have emphasized the advantages of three dimensional (3D) culture over the two dimensional (2D) culture of cells. In this study, thermos...
متن کاملMonologism of Hofstede’s Static Model vs Dialogism of Fang’s Dynamic Model: Contradictory Value Configuration of Cultures through the Case Study of Farsi Proverbs
Among various cultural models, the dichotomy of static versus dynamic models has provided a fertile ground for research. Although a number of static models are suggested, the dominant trend in almost all static models is provided by Hofstede who focuses on cultural differences along four major dimensions (power distance, individualism, uncertainty avoidance, and masculinity) and reduces “the co...
متن کاملHypoxia in static and dynamic 3D culture systems for tissue engineering of bone.
Tissue engineering of sizeable cell-scaffold constructs is limited by gradients in tissue quality from the periphery toward the center. Because homogenous delivery of oxygen to three-dimensional (3D) cell cultures remains an unsolved challenge, we hypothesized that uneven oxygen supply may impede uniform cellular growth on scaffolds. In this study we challenged static and dynamic 3D culture sys...
متن کاملInfluence of Different Three-Dimensional Open Porous Titanium Scaffold Designs on Human Osteoblasts Behavior in Static and Dynamic Cell Investigations
In the treatment of osseous defects micro-structured three-dimensional materials for bone replacement serve as leading structure for cell migration, proliferation and bone formation. The scaffold design and culture conditions are crucial for the limited diffusion distance of nutrients and oxygen. In static culture, decreased cell activity and irregular distribution occur within the scaffold. Dy...
متن کامل